Recombinant protein quality evaluation: proposal for a minimal information standard
نویسندگان
چکیده
The Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Faculty of Medicine, Nursing and Health Sciences, Monash University, Australia Monash eResearch Centre, Monash University, Clayton, Victoria, Australia Cogentech, Protein Chemistry Unit, Milan, Italy Max Planck Institute of Biochemistry, Department of Structural Cell Biology, Martinsried, Germany Protein Expression Laboratory, SAIC-Frederick Inc., National Cancer Institute, Frederick, MD USA Structural Genomics Consortium, Karolinska Institutet, Department of Medical Biophysics and Biochemistry, Stockholm, Sweden IEO, Milan, Italy Brown University, Department of Molecular Biology, Cell Biology and Biochemistry, Providence, RI, USA Max Planck Institute of Biochemistry, Microchemistry Core Facility, Martinsried, Germany National Institute for Biological Standards and Control, Health Protection Agency, Hertfordshire, UK Protein Technologies Group, Babraham Bioscience Technologies, Cambridge UK The European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, UK. Institute for Biotechnology and Biomedicine and Department of Genetics and Microbiology, Universitat Autònoma de Barcelona, and CIBER de Bioingeniería, Biomateriales y Nanomedicina (CIBER-BBN), Barcelona, Spain Department Environmental Sciences, University of Nova Gorica, Nova Gorica, Slovenia.
منابع مشابه
Minimal information: an urgent need to assess the functional reliability of recombinant proteins used in biological experiments
Structural characterization of proteins used in biological experiments is largely neglected. In most publications, the information available is totally insufficient to judge the functionality of the proteins used and, therefore, the significance of identified protein-protein interactions (was the interaction specific or due to unspecific binding of misfolded protein regions?) or reliability of ...
متن کاملHigh-level expression and evaluation of the antigenicity of a recombinant Toxoplasma gondii GRA2 Protein
متن کامل
Cloning and expression of fragment of the rabies virus nucleoprotein gene in Escherichia coli and evaluation of antigenicity of the expression product
Rabies virus nucleoprotein (N protein) encapsidates genomic RNA of the virus and forms the viral ribonucleoprotein complex. These N proteins represent highly organized structures which activate proliferation of B cells and production antibodies against the N protein. In addition to the B cell, the rabies virus N protein has been shown to induce potent T helper cell responses resulting in a long...
متن کاملImmunogenic evaluation of chimeric recombinant protein against ETEC, EHEC and Shigella
Diarrheal diseases still remain health problem worldwide and out of many bacteria responsible for, Shigella and pathogenic Escherichia cause the most diarrheas in the world. Shigellacause bacterial dysenteries and shigellosis through invasion where the most effective proteins for pathogenesis is Ipac. Critical virulence protein for ETEC infection is CFA/I with two subunits called cfab and cfae....
متن کاملExpansion of a highly sensitive and specific ELISA test for diagnosis of hydatidosis using recombinant EgB8/2 protein
Objective(s): Hydatidosis is a zoonotic infection and endemic in Iran. Due to the serological cross-reactivity (of sera) with other parasitic infection, diagnosis of hydatid cyst is considered to be problematic. In this regard, application of recombinant antigens improves serological diagnosis for human hydatidosis. Here, we present an ELISA test based on B8/2 recombinant antigen of Echinococcu...
متن کامل